Journal of Experimental Botany 62: 5131–5147 (2011)
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Members of the gibberellin receptor gene family GID1 (GIBBERELLIN INSENSITIVE DWARF1) play distinct roles during Lepidium sativum and Arabidopsis thaliana seed germination [W][OA]
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Antje Voegele, Ada Linkies, Kerstin Müller*, Gerhard Leubner-Metzger
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University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de
*Department of Biological Sciences, Simon Fraser University, 9 8888, University Drive, Burnaby BC, V5A 1S6, Canada
Received 14 March 2011; Revised 22 May 2011; Accepted 13 June 2011
Advance Acess publication 21 June 2011
DOI 10.1093/jxb/err214
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Supplemental Table S3. Primer sequences that were used for the quantitative PCR analyses.
Amplified
DNA |
Primer
Specificity |
Accession
number |
forward
Primer (5'-3') |
reverse
Primer (5'-3') |
LesaGID1a |
Lepidium
sativum |
HQ003455
(At3g05120) |
AGCGGGTGAGTCTGGAATCAAT |
AAGTTTCTCAGACTCTGTTCTC |
LesaGID1b |
Lepidium
sativum |
HQ003456
(At3g63010) |
AGCTCACAATGTTGCTGTGAG |
TCAGTCCTCTCTTGTCCAC |
LesaGID1c |
Lepidium
sativum |
HQ003457
(At5g27320) |
GCATAATGTCGCGGTTAGAG |
GTCCCTCCAAACATAGGGTT |
AtGID1a |
Arabidopsis
thaliana |
At3g05120 |
AGAGACCGCGATTGGTACTG |
TCGGGCTAAACGGATTACAC |
AtGID1b |
Arabidopsis
thaliana |
At3g63010 |
GGATCGGTTTGTTTTGCCTA |
TTATGCTTCCGGAGACCAAG |
AtGID1c |
Arabidopsis
thaliana |
At5g27320 |
AAGAGCGGTTGAGTCAAGGA |
TTCCCATCCAAACGTTTCTC |
EXLA1 |
Lepidium
sativum |
HO208805 |
ATGGCTAAGCCTGTTCCTGGTGCT |
TCCCGTAGTCGCAAGGAACTCTGT |
EXPA9 |
Lepidium
sativum |
HO208871 |
ATGTCAACGACGGTGAAGGCCA |
ATAGTGCCTTGAGCATCAGCCCCA |
XTH18 |
Lepidium
sativum |
HO208883 |
TGGGGGTTCCATGTGATGCAGT |
GGCTCAGGAGACAAAGAACAACAGT |
XTH19 |
Lepidium
sativum |
HO208914 |
GGGGGCACAGAGAAACTACATGGT |
TTAGCTGCACTCTGGAGGAACACCAC |
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