Journal of Experimental Botany 62: 5131–5147 (2011)

Members of the gibberellin receptor gene family GID1 (GIBBERELLIN INSENSITIVE DWARF1) play distinct roles during Lepidium sativum and Arabidopsis thaliana seed germination [W][OA]

Antje Voegele, Ada Linkies, Kerstin Müller*, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place'
*Department of Biological Sciences, Simon Fraser University, 9 8888, University Drive, Burnaby BC, V5A 1S6, Canada

Received 14 March 2011; Revised 22 May 2011; Accepted 13 June 2011
Advance Acess publication 21 June 2011
DOI 10.1093/jxb/err214

GID1 Lepidium seeds

Fig. 5. Transcript abundance patterns of the LesaGID1a, LesaGID1b, and LesaGID1c receptor genes in specific seed tissues of Lepidium sativum during germination.

A, Time course analysis of testa and endosperm rupture of L. sativum FR14 seeds in medium without (CON) and with ABA added. Continuous light, 18ºC. Mean values ± SE of 3x 50 seeds are presented. Red arrows indicate sampling time points for the qPCR.

B, Cross-section of L. sativum seed indicating the specific seed tissues used in the experiment: micropylar endosperm and radicle.

C,D, qRT-PCR analysis of LesaGID1a, LesaGID1b, and LesaGID1c transcript abundances for micropylar endosperm (C) and radicles (D) at 8, 18, and 96 h imbibition. ddCt expression values relative to validated constitutive transcripts are presented. Medium additions, as indicated: 10µM GA4+7, 10µM ABA. Mean values ± SE of 4x 1000 micropylar endosperms and 4x 200 radicles are presented; n.d.= not detectable.

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