New Phytologist 184: 885-897 (2009)

The NADPH-Oxidase AtrbohB plays a role in Arabidopsis seed after-ripening [W]

Kerstin Müller, Anna Catharina Carstens, Ada Linkies, Miguel Angel Torres, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' (K.M., A.C.C., A.L., G.L.-M.)
Centro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, 28223 Pozuelo de Alarcón (Madrid), Spain (M.A.T.)

Received April 30, 2009; accepted July 13, 2009; published online September 15, 2009
DOI 10.1111/j.1469-8137.2009.03005.x

Fig. 2 Mueller et al. 2009

Figure 2.
The Arabidopsis enhancer trap Line CS24365 (insertion in AtrbohB) exhibts an after-ripening phenotype: the ABA sensitivity of endosperm rupture does not change during after-ripening. (a) and (b) Germination kinetics of fresh (a) and after-ripened (ar) (b) seeds of WT (squares) and CS24365 (triangles) in the presence of 1 µM ABA. The black line indicates 50 % endosperm rupture, the dotted line connects it to the corresponding times t50% ( CS24365), the dashed line to t50% (WT). t50% represents the time needed to reach 50 % endosperm rupture. Means +/- SE of three times 50 seeds germinated at 24°C in continuous light are shown. (c) Comparison of t50%-values of fresh and after-ripened seeds for WT and CS24365 (AtrbohB insertion mutant). Ratios of t50% (after-ripened) / t50% (fresh) are given below the x-axis.


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