Journal of Experimental Botany 62: 5131–5147 (2011)
Members of the gibberellin receptor gene family GID1 (GIBBERELLIN INSENSITIVE DWARF1) play distinct roles during Lepidium sativum and Arabidopsis thaliana seed germination [W][OA]
*Department of Biological Sciences, Simon Fraser University, 9 8888, University Drive, Burnaby BC, V5A 1S6, Canada
Received 14 March 2011; Revised 22 May 2011; Accepted 13 June 2011
Advance Acess publication 21 June 2011
DOI 10.1093/jxb/err214
Supplemental Table S2. cDNA clones obtained of the early micropylar endosperm SSH library.
Putative gene function was based on the highest BLAST hit, usually Arabidopsis if not indicated otherwise. Based on the data of Ogawa et al. (2003) and Preston et al. (2009) GA-induction during seed germination of A. thaliana Ler was determined by eNorthern (Toufighi et al., 2005) using the BAR website (http://bar.utoronto.ca/). Transcripts with a ratio of at least 1.5 between GA and control treatments at least one ot the three time points (3h, 6h, 9h) were considered as GA-induced ("Yes").
Clone number |
GenBank
|
Locus
of closest |
Putative gene product / function |
Sequence
similarity to Arabidopsis (%) , |
GA-induciblity |
pUC19-8h23 |
HO208984 |
At1g08110 |
lactoylgluthatione lyase / glyoxylase1 |
91.8 |
No |
8h-151 |
HO208845 |
At1g08770 |
PRA1 (prenylated rab acceptor) |
80.2 |
No |
8h-106 |
HO208806 |
At1g11350 |
universal stress protein (USP) |
87.4 |
Yes |
8h-38 |
HO208897 |
At1g15690 |
AVP1 (vacuolar-type H+-pumping pyrophosphatease 1) |
96.2 |
Yes |
8h-93 |
HO208946 |
At1g17620 |
unknown protein |
83.8 |
Yes |
pTopo-8h19 |
HO208958 |
At1g17720 |
serine/threonine protein phosphatase |
72.5 |
Yes |
8h-17 |
HO208861 |
At1g18210 |
calcium-binding |
87.9 |
No |
8h-54 |
HO208913 |
At1g18540 |
60S ribosomal protein L6 (RPL6A) |
84.8 |
No |
8h-69 |
HO208923 |
At1g18540 |
60S ribosomal protein L6 (RPL6A) |
90.6 |
No |
8h-104 |
HO208804 |
At1g19770 |
PUP14 (purin permease) |
86.8 |
No |
8h-176 |
HO208864 |
At1g23010 |
multi-copper oxidase type I family |
82.0 |
No |
pTopo-8h13 |
HO208953 |
At1g30270 |
CIPK23 (CBL-interacting protein kinase ) |
94.4 |
Yes |
8h-162 |
HO208854 |
At1g30690 |
SEC14 (cytosolic factor) |
87.1 |
Yes |
8h-31 |
HO208891 |
At1g31850 |
dehydration-responsive |
91.7 |
No |
8h-189 |
HO208876 |
At1g47128 |
cysteine proteinase RD21A |
89.5 |
Yes |
8h-56 |
HO208915 |
At1g36280 |
adenylosuccinate lyase |
93.1 |
Yes |
8h-158 |
HO208851 |
At1g53510 |
MPK18 (map kinase) |
88.2 |
No |
8h-187 |
HO208874 |
At1g56090 |
tetratricopeptide repeat (TPR)-containing protein |
85.3 |
No |
8h-52 |
HO208911 |
At1g56300 |
DNAJ heat shock N-terminal domain-containing |
86.2 |
No |
8h-141 |
HO208835 |
At1g60160 |
potassium transporter family protein |
93.2 |
Yes |
8h-111 |
HO208811 |
At1g63000 |
RHM1/ROL1 (rhamnose biosynthesis) |
87.7 |
Yes |
8h-149 |
HO208842 |
At1g63000 |
RHM1/ROL1 (rhamnose biosynthesis) |
86.6 |
No |
8h-44 |
HO208902 |
At1g65930 |
isocitrate dehydrogenase |
93.4 |
No |
8h-129 |
HO208824 |
At1g64650 |
unknown protein |
87.6 |
No |
8h-185 |
HO208872 |
At1g64650 |
unknown protein |
87.5 |
No |
pTopo-8h2 |
HO208959 |
At1g69910 |
protein kinase family protein |
85.3 |
No |
8h-2 |
HO208881 |
At1g70090 |
GATL9 (galacturonosyltransferase-like) |
89.7 |
No |
8h-130 |
HO208825 |
At1g71920 |
histidinol-phosphate aminotransferase |
92.1 |
No |
8h-91 |
HO208944 |
At1g72510 |
unknown protein |
83.1 |
Yes |
8h-182 |
HO208870 |
At1g79850 |
Ribosomal protein S17 |
77.2 |
No |
8h-171 |
HO208862 |
At1g80120 |
unknown protein |
86.2 |
No |
pUC19-8h11 |
HO208975 |
At2g05220 |
40S ribosomal protein S17 (RPS17B) |
85.3 |
No |
pTopo-8h9 |
HO208970 |
At2g05755 |
integral membrane family protein |
80.4 |
Yes |
8h-16 |
HO208852 |
At2g05929 |
subtilisin serine protease |
87.7 |
No |
8h-96 |
HO208948 |
At2g16365 |
F-box family |
85.9 |
No |
8h-8 |
HO208932 |
At2g17420 |
NADH-dependent thioredoxin reductase 2 |
79.5 |
No |
Puc19-19 |
HO208981 |
At2g17930 |
inositol or phosphatidylinositol kinase/ phosphotransferase |
94.1 |
No |
8h-48 |
HO208906 |
At2g22090 |
UBA1 (ubiquitin activating enzyme) |
90.0 |
No |
8h-160 |
HO208853 |
At2g22500 |
mitochondrial dicarboxylate carrier protein |
73.7 |
Yes |
8h-18 |
HO208867 |
At2g23310 |
RERC1C1 (endoplasmatic reticulum retrieval 1C) |
79.1 |
No |
8h-163 |
HO208855 |
At2g24280 |
serine carboxypeptidase S28 family |
89.0 |
No |
8h-153 |
HO208847 |
At2g28190 |
CSD2 (copper/zinc SOD) |
93.1 |
No |
8h-29 |
HO208889 |
At2g32070 |
CCR4-NOT transcription complex protein |
91.4 |
No |
8h-135 |
HO208829 |
At2g32070 |
CCR4-NOT transcription complex protein |
91.7 |
No |
8h-9 |
HO208943 |
At2g34250 |
sec61 (protein transport) |
95.4 |
No |
8h-128 |
HO208823 |
At2g34480 |
60S ribosomal protein L18A |
90.5 |
No |
8h-51 |
HO208910 |
At2g36460 |
putative fructose biphosphate aldolase |
88.2 |
No |
8h-79 |
HO208931 |
At2g36460 |
putative fructose biphosphate aldolase |
88.1 |
No |
pTopo-8h23 |
HO208962 |
At2g36460 |
putative fructose biphosphate aldolase |
88.0 |
No |
pTopo-8h3 |
HO208964 |
At2g37640 |
EXPA3 (expansin) |
76.5 |
Yes |
pTopo-8h5 |
HO208966 |
At2g37640 |
EXPA3 (expansin) |
76.5 |
No |
pTopo-8h12 |
HO208952 |
At2g37640 |
EXPA3 (expansin) |
81.8 |
No |
8h-166 |
HO208857 |
At2g38710 |
AMMECR1 family |
91.6 |
No |
8h-191 |
HO208879 |
At2g39290 |
PGPS 1 (phosphatidylglycerolphosphate synthase 2C) |
91.4 |
No |
8h-53 |
HO208912 |
At2g40000 |
putative nematode resistant |
90.7 |
Yes |
8h-119 |
HO208814 |
At2g40330 |
Bet vI allergen family; abscisic acid receptor PYL6 |
80.7 |
Yes |
pUC19-8h22 |
HO208983 |
At2g41900 |
zinc-finger (CCCH-type) family |
89.3 |
No |
pTopo-8h21 |
HO208961 |
At2g42700 |
unknown protein |
89.3 |
No |
pTopo-8h24 |
HO208963 |
At2g42700 |
unknown protein |
85.3 |
No |
8h-71 |
HO208924 |
At2g43160 |
epsin N-terminal homology domain-containing |
93.2 |
No |
8h-45 |
HO208903 |
At2g46330 |
AGP16 (arabinogalactan protein 16) |
80.1 |
Yes |
8h-28 |
HO208888 |
At2g47640 |
small nuclear riboonucleoprotein D2 |
87.7 |
No |
8h-72 |
HO208925 |
At2g47640 |
small nuclear riboonucleoprotein D2 |
86.9 |
No |
8h-39 |
HO208898 |
At3g01360 |
unknown protein |
85.6 |
No |
8h-188 |
HO208875 |
At3g02880 |
leucine-rich repeat transmembrane protein kinase |
78.9 |
Yes |
pUC19-8h14 |
HO208978 |
At3g03570 |
expressed protein |
91.1 |
Yes |
8h-169 |
HO208860 |
At3g03640 |
Beta glucosidase 25 |
89.4 |
No |
8h-137 |
HO208831 |
At3g03710 |
RIF10 (resistant to inhibition with FSM 10) |
81.6 |
No |
8h-165 |
HO208856 |
At3g05880 |
RCI2A (rare cold inducible) |
77.2 |
No |
pUC19-8h12 |
HO208976 |
At3g07390 |
plant IF-like protein |
77.1 |
Yes |
8h-19 |
HO208877 |
At3g08030 |
unknown protein |
89.1 |
No |
8h-150 |
HO208844 |
At3g09630 |
60S ribosomal protein L1 |
80.5 |
No |
8h-192 |
HO208880 |
At3g10930 |
unknown protein |
74.2 |
Yes |
pTopo-8h7 |
HO208968 |
At3g10980 |
Senescence associated gene 20 SAG20 |
87.6 |
No |
8h-122 |
HO208817 |
At3g11400 |
EIF3G (translation initiation factor) |
88.8 |
No |
8h-89 |
HO208942 |
At3g11510 |
40S ribosomal protein S14 (RPS14B) |
87.7 |
No |
8h-177 |
HO208865 |
At3g14090 |
unknown protein |
87.4 |
No |
Puc19-8h16 |
HO208979 |
At3g16270 |
unknown protein |
71.6 |
No |
8h-12 |
HO208815 |
At3g16640 |
TCTP (translationally controlled tumor) |
80.3 |
No |
8h-26 |
HO208886 |
At3g16640 |
TCTP (translationally controlled tumor) |
88.1 |
No |
8h-84 |
HO208937 |
At3g18190 |
chaperonin |
83.1 |
No |
8h-190 |
HO208878 |
At3g19920 |
unknown protein |
85.3 |
No |
8h-145 |
HO208838 |
At3g20898 |
unknown protein |
83.1 |
No |
Puc19-8h10 |
HO208974 |
At3g21720 |
isocitrate lyase |
94.0 (B. napus) |
No |
8h-110 |
HO208810 |
At3g21890 |
zinc-finger (B-box type) family |
74.0 |
Yes |
8h-181 |
HO208869 |
At3g24830 |
60S ribosomal protein L13A (RPL13aB) |
91.9 |
No |
8h-143 |
HO208837 |
At3g25040 |
HDEL ER lumen protein |
82.0 |
Yes |
8h-105 |
HO208805 |
At3g45970 |
EXLA1 (expansin-like) |
85.2 |
No |
pTopo-8h14 |
HO208954 |
At3g48140 |
senescence-associated |
88.9 |
Yes |
8h-62 |
HO208917 |
At3g49720 |
unknown protein |
89.6 |
Yes |
8h-174 |
HO208863 |
At3g52580 |
40S ribosomal protein S14 (RPS14C) |
87.8 |
No |
8h-179 |
HO208866 |
At3g55440 |
triose-phosphate isomerase (TPI) |
91.3 |
No |
8h-146 |
HO208839 |
At3g57090 |
binding (BIGYIN) |
84.3 |
No |
Puc19-8h7 |
HO208988 |
At3g57520 |
putative imbibition protein homolog |
91.3 |
Yes |
8h-64 |
HO208972 |
At3g57550 |
AGK2 (guanylate-kinase) |
91.1 |
Yes |
8h-99 |
HO208949 |
At3g59770 |
SAC 9 (suppressor of actin) |
90.4 |
No |
Puc19-8h20 |
HO208982 |
At3g63030 |
MBD4 (mathyl-CpG-binding domain 4, DNA-binding) |
83.2 |
No |
8h-107 |
HO208807 |
At4g00730 |
transcription factor/ transcription regulator (Anthocyanless 2) |
74.1 |
No |
8h-76 |
HO208929 |
At4g05050 |
UBQ11 (ubiquitin) |
88.2 |
Yes |
pTopo-8h15 |
HO208955 |
At4g11740 |
SAY1 (Isolated as a suppressor of a dominant mutant in the Ara4 gene ) |
80.8 |
No |
8h-136 |
HO208830 |
At4g13010 |
oxidoreductase, zinc-binding dehydrogenase family |
85.2 |
No |
8h-133 |
HO208827 |
At4g13940 |
HOG1 (homology-dependent gene silencing, adenosylhomocysteinase) |
93.6 |
No |
8h-67 |
HO208921 |
At4g15470 |
unknown protein |
80.7 |
No |
pUC19-8h18 |
HO208980 |
At4g15470 |
unknown protein |
90.8 |
No |
8h-35 |
HO208894 |
At4g15530 |
PPDK (pyruvate orthophosphate dikinase) |
92.9 |
Yes |
8h-78 |
HO208930 |
At4g16563 |
aspartyl protease family |
87.4 |
No |
8h-127 |
HO208822 |
At4g17420 |
unknown protein |
92.5 |
No |
8h-27 |
HO208887 |
At4g18650 |
transcription-factor related |
86.0 |
Yes |
8h-87 |
HO208940 |
At4g20860 |
FAD-binding domain |
90.0 |
No |
8h-88 |
HO208941 |
At4g20860 |
FAD-binding domain |
90.0 |
No |
8h-102 |
HO208802 |
At4g22820 |
AN1-like zinc finger family |
79.5 |
No |
8h-156 |
HO208849 |
At4g24220 |
VEP1 (vein patterning) |
94.1 |
No |
8h-5 |
HO208908 |
At4g24770 |
RBP31 (RNA binding) |
76.8 |
No |
8h-74 |
HO208927 |
At4g26000 |
PEP (nucleic acid binding) |
91.0 |
No |
8h-101 |
HO208801 |
At4g27070 |
TSB2 (tryptophane synthase beta subunit) |
82.5 |
No |
pTopo-8h1 |
HO208950 |
At4g27320 |
universal stress protein (USP) |
81.8 |
Yes |
8h-6 |
HO208916 |
At4g27652 |
unknown protein |
73.7 |
No |
8h-138 |
HO208832 |
At4g28440 |
DNA-binding protein-related |
90.1 |
No |
8h-22 |
HO208884 |
At4g29130 |
HXK1 (hexokinase, glucose insensitive 1) |
93.9 |
Yes |
8h-75 |
HO208928 |
At4g29390 |
40S ribosomal protein S30 (RPS30B) |
91.5 |
No |
8h-36 |
HO208895 |
At4g30270 |
MERI5B (xyloglucan endo-1,4-Betaglucanase) |
89.3 |
No |
8h-21 |
HO208883 |
At4g30280 |
XTH18 |
86.9 |
Yes |
8h-46 |
HO208904 |
At4g30280 |
XTH18 |
87.4 |
Yes |
8h-47 |
HO208905 |
At4g30280 |
XTH18 |
87.4 |
Yes |
8h-131 |
HO208826 |
At4g30280 |
XTH18 |
87.4 |
Yes |
pTopo-8h6 |
HO208967 |
At4g30280 |
XTH18 |
85.7 |
Yes |
8h-55 |
HO208914 |
At4g30290 |
XTH19 |
80.6 |
Yes |
8h-25 |
HO208885 |
At4g31130 |
unknown protein |
85.7 |
No |
Puc19-8h1 |
HO208973 |
At4g32020 |
unknown protein |
69.6 |
No |
8h-73 |
HO208926 |
At4g32130 |
unknown protein |
72.5 |
No |
8h-86 |
HO208939 |
At4g32390 |
phosphate-translocator-related |
90.4 |
No |
8h-95 |
HO208947 |
At4g34555 |
40S ribosomal protein S25 |
81.8 |
Yes |
8h-152 |
HO208846 |
At4g37580 |
HLS1 (hookless, N-acetyltransferase) |
85.6 |
Yes |
8h-34 |
HO208893 |
At4g38740 |
ROC1 (peptidylprolyl isomerase) |
89.5 |
No |
8h-20 |
HO208882 |
At4g38800 |
MTN1 (methylthioadenosin nucleosidase) |
89.4 |
No |
8h-83 |
HO208936 |
At5g01320 |
pyruvate decarboxylase |
83.4 |
Yes |
8h-167 |
HO208858 |
At5g01710 |
unknown protein |
89.0 |
No |
8h-139 |
HO208833 |
At5g02260 |
EXPA9 (expansin A9) |
78.2 |
Yes |
8h-184 |
HO208871 |
At5g02280 |
ATEXPA9 (ARABIDOPSIS THALIANA EXPANSIN A9) (ATEXPA9) |
78.4 |
No |
8h-49 |
HO208907 |
At5g02610 |
60S ribosomal protein L35 (RPL35D) |
84.3 |
No |
8h-50 |
HO208909 |
At5g02610 |
60S ribosomal protein L35 (RPL35D) |
84.3 |
No |
pTopo-8h17 |
HO208957 |
At5g03040 |
calmodulin binding (iqd2); (IQ-domain 2) |
89.5 |
Yes |
8h-164 |
HO208971 |
At5g03880 |
electron carrier |
78.3 |
No |
8h-40 |
HO208899 |
At5g07340 |
Calnexin |
83.0 |
No |
pTopo-8h20 |
HO208960 |
At5g07830 |
Beta-glucuronidase AtGUS2 |
91.2 |
No |
8h-103 |
HO208803 |
At5g08160 |
PK3 serine/threonine protein kinase |
93.2 |
No |
Puc19-8h4 |
HO208987 |
At5g10750 |
unknown protein |
87.6 |
No |
8h-30 |
HO208890 |
At5g10770 |
chloroplast nucleoid DNA-binding protein |
82.6 |
Yes |
8h-157 |
HO208850 |
At5g10860 |
CBS domaine containing |
84.1 |
No |
8h-41 |
HO208900 |
At5g11090 |
serine-rich protein-related |
81.2 |
No |
8h-123 |
HO208818 |
At5g12140 |
CYS1 (cystatin) |
83.4 |
No |
8h-63 |
HO208918 |
At5g12850 |
zinc-finger (CCCH-type) family |
89.6 |
Yes |
8h-112 |
HO208812 |
At5g14450 |
GDSL-motif lipase/hydrolase family |
90.1 |
Yes |
8h-148 |
HO208841 |
At5g15650 |
RGP2 (reversibly glycosylated polypeptide-3) |
90.8 |
Yes |
pTopo-8h16 |
HO208956 |
At5g15850 |
COL1 (constance-like) |
86.0 |
Yes |
8h-147 |
HO208840 |
At5g17860 |
CAX7 (calcium exchanger) |
89.3 |
Yes |
8h-134 |
HO208828 |
At5g17920 |
CIMS (cobalamin-independent methionine synthase) |
93.2 |
Yes |
Puc19-8h9 |
HO208989 |
At5g20290 |
40S ribosomal protein S8 (RPS8A) |
81.0 |
No |
8h-168 |
HO208859 |
At5g25460 |
Unknown protein |
93.9 |
No |
8h-33 |
HO208892 |
At5g28060 |
40S ribosomal protein S24 (RPS24B) |
78.9 |
No |
8h-42 |
HO208901 |
At5g29560 |
Calcium-ion-binding |
89.0 |
No |
pTopo-8h4 |
HO208965 |
At5g35160 |
Unknown protein |
85.2 |
No |
8h-82 |
HO208935 |
At5g35460 |
unknown protein |
89.9 |
No |
8h-120 |
HO208816 |
At5g35560 |
MOK9_2 |
86.6 |
No |
8h-85 |
HO208938 |
At5g38890 |
exoribonuclease-related |
91.1 |
Yes |
8h-116 |
HO208813 |
At5g43940 |
ADH2 alcohol dehydrogenase |
92.2 |
No |
Puc19-8h3 |
HO208986 |
At5g44420 |
PDF1.2c (plant defensin 1.2c) (PDF1.2c) |
81.3 |
No |
8h-68 |
HO208922 |
At5g45280 |
pectinacetylesterase |
90.4 |
Yes |
Puc19-8h24 |
HO208985 |
At5g45775 |
60S ribosomal protein L11 (RPL11D) |
92.8 |
No |
8h-65 |
HO208919 |
At5g48545 |
unknown protein |
88.6 |
No |
8h-125 |
HO208820 |
At5g51570 |
band 7 family protein |
83.1 |
No |
8h-180 |
HO208868 |
At5g53570 |
RabGAP/TBC domain-containing protein |
84.1 |
No |
8h-142 |
HO208836 |
At5g54855 |
pollen allergene Ole e1 and extensin family |
90.2 |
No |
8h-186 |
HO208873 |
At5g55190 |
RAN3 (RAN GTPase gene family) |
93.0 |
No |
pTopo-8h8 |
HO208969 |
At5g56030 |
HSP81-2 (early-responsive to dehydration) |
91.2 |
Yes |
8h-109 |
HO208809 |
At5g60390 |
elongation factor 1-alpha |
85.6 |
No |
8h-66 |
HO208920 |
At5g61230 |
Ankyrin repeat family |
76.9 |
No |
8h-124 |
HO208819 |
At5g61820 |
unknown protein |
73.3 |
No |
8h-108 |
HO208808 |
At5g64270 |
splicing factor |
92.0 |
No |
8h-155 |
HO208848 |
At5g64310 |
Arabinogalactan protein 1 |
69.9 |
Yes |
8h-92 |
HO208945 |
At5g64400 |
unknown protein |
82.7 |
No |
8h-14 |
HO208834 |
At5g65140 |
trehalose-6-phosphate phosphatase |
92.0 |
Yes |
8h-15 |
HO208843 |
At5g66590 |
allergen V5/Tpx-1-related |
84.4 |
No |
8h-80 |
HO208933 |
At5g66590 |
allergen V5/Tpx-1-related |
82.9 |
No |
 |
|
|
|
|
|
| Article in PDF format (1.5 MB) Supplementary data file (6.5 MB) |
|
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