Plant Physiology 161: 1903-1917 (2013)

Spatiotemporal seed development analysis provides insight into primary dormancy induction and evolution of the Lepidium DELAY OF GERMINATION1 Genes [W][OA]

Kai Graeber, Antje Voegele, Annette BŁttner-Mainik, Katja Sperber, Klaus Mummenhoff, Gerhard Leubner-Metzger

School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (KG, AV, GLM);
Web: 'The Seed Biology Place' - www.seedbiology.eu
University of Freiburg, Faculty of Biology, Institute for Biology II, Botany/Plant Physiology, D-79104 Freiburg, Germany (KG, AV, ABM, GLM)
Universität Osnabrück, Fachbereich Biologie, Botanik, D-49069 Osnabrück, Germany (KS, KM)

Received December 24, 2012; Accepted Feburary 19, 2013; Published Feburary 20, 2013.
DOI:10.1104/pp.112.213298

Supplemental Table S1  Putative DOG1 orthologs cloned from diverse Lepidium species.

Lepidium species

GenBank accession number

Sequence type
[Gene name used in text]

Size (bp)

Primer paird

Sequence similarity (%)
to LesaDOG1a

Accession number

Origin

L. campestre

JX512181

genomic

1146

B

72.0

OSBU 96-0070-30-00

Greece, Thrace, Nomos Evrou, Korimvos

L. africanumc

JX512177

genomic

1323

B

66.3

OSBU 22265

Australia, Melbourne, Royal Park, south of Park Street; coll. N. Scarlett

L. pseudohyssopifoliumc

JX512179

genomic

1152

B

66.6

OSBU 22263

Australia Victoria, Mitre Rock, near Mt. Arapiles; 141°50`E 36°44`S;  coll. N. H. Scarlett

L. ruderalec

JX512180

genomic

1369

B

64.1

OSBU 05-0209-10-00

East Kazakhstan, Zaissan distr., Akzhan; coll. Antonyuk

L. bonariensec

JX512178

genomic

1238

B

71.8

OSBU 95-0022-10-00

Australia, NSW, Broken Hill; coll. H. Hurka

L. papillosum

JX512183

genomic
[LepaDOG1a]

1343

Ae

85.0

Mummenhoff 2415

Australia, NSW, 10 km west of Broken Hill

L. papillosum

JX512187

mRNA
[LepaDOG1a]

442

E

100.0

Mummenhoff 2415

dito

L. papillosum

JX512184

genomic
[LepaDOG1b]

1317

Be

73.5

Mummenhoff 2415

dito

L. papillosum

JX512185

genomic
[LepaDOG1c]

1583

Ce

65.6

Mummenhoff 2415

dito

L. papillosum

JX512188

mRNA
[LepaDOG1c]

386

F

90.4

Mummenhoff 2415

dito

L. papillosum

JX512186

genomic
[LepaDOG1d]

832

D

43.6

Mummenhoff 2415

dito

L. didymum

JX512176

genomic

1216

B

74.0

OSBU 22266

Germany, Osnabrück, near City Hall; coll. K. Sperber

L. leptopetalumb

JX512182

genomic

279

C

87.5

OSBU 22264

Australia, Victoria, near Carwarp; 142°06`E 34°38`S, coll. A.H. Brown


a Cloned genomic fragments have been aligned against genomic LesaDOG1 sequence; cloned cDNA sequences have been aligned against LesaDOG1 cDNA sequence. Similarity is the % pairwise identity within the aligned region. Global alignment was performed using MUSCLE. All genomic fragments cover at least partially exon 1 and exon 2 including the whole intron.
b This genomic fragment only covers exon 1.
c For these species also another smaller genomic fragment covering only exon 1 was cloned, which is not identical to the listed larger genomic fragment; this indicates the presence of multiple DOG1 genes in these species.
d Primer pair abbreviations refer to Supplemental Table S2.
e These primer pairs were used for the initial cloning of L. papillosum DOG1 gDNA fragments. To extend these sequences primers were designed based on an alignment of these gDNA sequences at highly divergent regions to obtain specific genomic fragments of the individual L. papillosum DOG1 genes. Consensus sequences of these genomic fragments were derived for each L. papillosum DOG1 gene.

Article in PDF format (2 MB)
Supplementary data file (4.9 MB)
Abstract

Fig. 1         Fig. 2         Fig. 3         Fig. 4         Fig. 5         Fig. 6         Fig. 7
Fig. S1       Fig. S2       Fig. S3      Fig. S4       Table S1    Table S2
 
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