Plant Physiology 161: 1903-1917 (2013)

Spatiotemporal seed development analysis provides insight into primary dormancy induction and evolution of the Lepidium DELAY OF GERMINATION1 Genes [W][OA]

Kai Graeber, Antje Voegele, Annette BŁttner-Mainik, Katja Sperber, Klaus Mummenhoff, Gerhard Leubner-Metzger

School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (KG, AV, GLM);
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University of Freiburg, Faculty of Biology, Institute for Biology II, Botany/Plant Physiology, D-79104 Freiburg, Germany (KG, AV, ABM, GLM)
Universität Osnabrück, Fachbereich Biologie, Botanik, D-49069 Osnabrück, Germany (KS, KM)

Received December 24, 2012; Accepted Feburary 19, 2013; Published Feburary 20, 2013.

LesaDOG1 construct

Supplemental Figure S3. Plasmid Map of modified pDEST15 vector containing a TEV cleavage site used for recombinant GST-LesaDOG1 fusion protein expression.

Predicted molecular masses of native GST and LesaDOG1 proteins are shown on the inside, predicted masses of GST* and LesaDOG1* proteins resulting from TEV cleavage of the fusion protein are shown on the outside of the plasmid map. GST-Linker(TEV)-LesaDOG1 is encoded as a continuous ORF on the plasmid. Numbers on plasmid: bp.

Article in PDF format (2 MB)
Supplementary data file (4.9 MB)

Fig. 1         Fig. 2         Fig. 3         Fig. 4         Fig. 5         Fig. 6         Fig. 7
Fig. S1       Fig. S2       Fig. S3      Fig. S4       Table S1    Table S2
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