Plant Molecular Biology 73: 67-87 (2010)

Cross-species approaches to seed dormancy and germination: conservation and biodiversity of ABA-regulated mechanisms and the Brassicaceae DOG1 genes

Kai Graeber • Ada Linkies • Kerstin Müller • Andrea Wunchova • Anita Rott • Gerhard Leubner-Metzger

Botany / Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' (K.G., A.L., K.M., A.R., G.L.-M.)
Department of Cell Biology and Genetics, Palacky University in Olomouc, Slechtitelu 11, 78371 Olomouc-Holice, Czech Republic, and Department of Botany and Plant Physiology, Mendel University of Agriculture and Forestry in Brno, Zemedelska 1, 61300 Brno, Czech Republic (A.W.)

Received August 28, 2009; Accepted November 22, 2009; Published online: December 15, 2009
DOI: 10.1007/s11103-009-9583-x

Species Geographical origin Dormancy of accession Cold stratification Seed batch size
Lepia s.l. (major clade)
L. draba EA ND + L
L. campestre EA ND + L
Lepidium s.s. (major clade)
L. africanum AF ND n.d. S
L. divaricatum AF ND - L
L. hyssopifolium AU+NZ ND n.d. S
L. pseudohyssopifolium AU ND n.d. S
L. monoplocoides AU ND n.d. S
L. oxytrichum AU CD* + L
L. papillosum AU ED n.d. S
L. didymus SA CD + L
L. bonariense SA CD + L
L. spinosum EA ND n.d. S
L. sativum FR14 EA ND* - L
L. ruderale EA ND + L
Monoploca s.s. (major clade)
L. rotundum AU ED n.d. S
L. pedicellosum AU ED* - L
L. phlebopetalum AU ED n.d. S
L. pholidogynum AU ED* - L

Table 1. Screening of Lepidium spp. ('cress') accessions for dormancy and germination characteristics.
Seed populations were considered as non-dormant (ND) if they germinated under control conditions (CON = 24ºC, continuous light, medium without hormone addition). Coat dormant (CD) seeds germinated after a scarification treatment (cutting or removal of the seed covering layers). Seed populations designated as embryo dormant (ED) did not germinate, also not after scarification;
*Embryo viability was confirmed by a tetrazolium test (see Fig. 3C).
'+' means that cold stratification (incubation of imbibed seeds in darkness at 4ºC for 48 h) caused dormancy release and/or promoted germination, '-' no effect, n.d. = not determined.
Geographic origin of the species: EA = Europe + Asia, AF = Africa, AU = Australia, NZ = New Zealand, SA = South America. Geographical grouping of the species largely resembles phylogenetic relationship.
Seed batch sizes: L = Large seed batch used for screening, 1-3 plates with 50 seeds each were analysed (mean values ± SE in Fig. 3); S = Small seed batch used for screening, usually one plate with 10-30 seeds was analysed.

Article in PDF format (872 KB)
Abstract of PMB 2009  
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