Plant Molecular Biology 73: 67-87 (2010)
Cross-species approaches to seed dormancy and germination: conservation and biodiversity of ABA-regulated mechanisms and the Brassicaceae DOG1 genes
Botany / Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de (K.G., A.L., K.M., A.R., G.L.-M.)
Department of Cell Biology and Genetics, Palacky University in Olomouc, Slechtitelu 11, 78371 Olomouc-Holice, Czech Republic, and Department of Botany and Plant Physiology, Mendel University of Agriculture and Forestry in Brno, Zemedelska 1, 61300 Brno, Czech Republic (A.W.)
Received August 28, 2009; Accepted November 22, 2009; Published online: December 15, 2009
DOI: 10.1007/s11103-009-9583-x
| Species | Geographical origin | Dormancy of accession | Cold stratification | Seed batch size |
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| Lepia s.l. (major clade) |
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| L. draba | EA | ND | + | L |
| L. campestre | EA | ND | + | L |
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| Lepidium s.s. (major clade) |
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| L. africanum | AF | ND | n.d. | S |
| L. divaricatum | AF | ND | - | L |
| L. hyssopifolium | AU+NZ | ND | n.d. | S |
| L. pseudohyssopifolium | AU | ND | n.d. | S |
| L. monoplocoides | AU | ND | n.d. | S |
| L. oxytrichum | AU | CD* | + | L |
| L. papillosum | AU | ED | n.d. | S |
| L. didymus | SA | CD | + | L |
| L. bonariense | SA | CD | + | L |
| L. spinosum | EA | ND | n.d. | S |
| L. sativum FR14 | EA | ND* | - | L |
| L. ruderale | EA | ND | + | L |
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| Monoploca s.s. (major clade) |
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| L. rotundum | AU | ED | n.d. | S |
| L. pedicellosum | AU | ED* | - | L |
| L. phlebopetalum | AU | ED | n.d. | S |
| L. pholidogynum | AU | ED* | - | L |
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Table 1. Screening of Lepidium spp. ('cress') accessions for dormancy and germination characteristics.
Seed populations were considered as non-dormant (ND) if they germinated under control conditions (CON = 24ºC, continuous light, medium without hormone addition). Coat dormant (CD) seeds germinated after a scarification treatment (cutting or removal of the seed covering layers). Seed populations designated as embryo dormant (ED) did not germinate, also not after scarification;
*Embryo viability was confirmed by a tetrazolium test (see Fig. 3C).
'+' means that cold stratification (incubation of imbibed seeds in darkness at 4ºC for 48 h) caused dormancy release and/or promoted germination, '-' no effect, n.d. = not determined.
Geographic origin of the species: EA = Europe + Asia, AF = Africa, AU = Australia, NZ = New Zealand, SA = South America. Geographical grouping of the species largely resembles phylogenetic relationship.
Seed batch sizes: L = Large seed batch used for screening, 1-3 plates with 50 seeds each were analysed (mean values ± SE in Fig. 3); S = Small seed batch used for screening, usually one plate with 10-30 seeds was analysed.
| Article in PDF format (872 KB) Abstract of PMB 2009 |
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