Plant Molecular Biology 73: 67-87 (2010)

Cross-species approaches to seed dormancy and germination: conservation and biodiversity of ABA-regulated mechanisms and the Brassicaceae DOG1 genes

Kai Graeber • Ada Linkies • Kerstin Müller • Andrea Wunchova • Anita Rott • Gerhard Leubner-Metzger

Botany / Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' (K.G., A.L., K.M., A.R., G.L.-M.)
Department of Cell Biology and Genetics, Palacky University in Olomouc, Slechtitelu 11, 78371 Olomouc-Holice, Czech Republic, and Department of Botany and Plant Physiology, Mendel University of Agriculture and Forestry in Brno, Zemedelska 1, 61300 Brno, Czech Republic (A.W.)

Received August 28, 2009; Accepted November 22, 2009; Published online: December 15, 2009
DOI: 10.1007/s11103-009-9583-x

Lepidium DOG1 expression ABA

Figure 6.  LesaDOG1 transcript expression pattern determined by qRT-PCR in specific Lepidium sativum FR1 seed tissues during incubation on medium without (CON) or with ABA added. Relative expression values based on the comparison with validated constitutive transcripts are presented.
(A) Micropylar seed ends (radicle plus endosperm cap) of dry (0h) and imbibed (8h ± ABA) seeds are compared; 8h-CON was set to 1. Note that tissue separation of dry seeds is not possible.
(B) Radicles and micropylar endosperm caps dissected from seeds at the times indicated were analysed; radicle 8h-CON was set to 1. Numbers above the columns are the percentage of endosperm rupture of the seed populations. Mean values +SE of 4 biological replicas; incubation of seeds at 24 ºC in continuous light, ± 10 µM ABA.

Article in PDF format (872 KB)
Abstract of PMB 2009  
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