Plant Molecular Biology 73: 67-87 (2010)
Cross-species approaches to seed dormancy and germination: conservation and biodiversity of ABA-regulated mechanisms and the Brassicaceae DOG1 genes
Botany / Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de (K.G., A.L., K.M., A.R., G.L.-M.)
Department of Cell Biology and Genetics, Palacky University in Olomouc, Slechtitelu 11, 78371 Olomouc-Holice, Czech Republic, and Department of Botany and Plant Physiology, Mendel University of Agriculture and Forestry in Brno, Zemedelska 1, 61300 Brno, Czech Republic (A.W.)
Received August 28, 2009; Accepted November 22, 2009; Published online: December 15, 2009
DOI: 10.1007/s11103-009-9583-x
Figure 4. Apoplastic hydroxyl radical (•OH) production in plant tissues. In addition to the ABA-GA antagonism (Müller et al. 2009) an ABA-ACC antagonism is evident indicative for a role of ethylene in counteracting the ABA inhibition on endosperm rupture during seed germination.
(A) Proposed hypotheses for the production of apoplastic •OH as described in the main text.
(B) In vivo detection of apoplastic •OH production by EPR in Lepidium sativum FR1 endosperm caps and radicles incubated for 40 h in medium with 5 µM ABA alone (ABA) or combined with 10 µM GA4+7 (ABA+GA) or 1 mM ACC (ABA+ACC); ACC (1-aminocyclopropane-1-carboxylic acid) is the direct precursor of ethylene. Means of radicle samples (ABA vs. ABA+GA and ABA vs. ABA+ACC) differ significantly (P<0.05) as calculated by one way ANOVA followed by Tukey’s multiple comparison test (GraphPad Prism software). For comparison the endosperm rupture values are given as numbers above the columns. Mean values +/- SE of at least four replicates of 100 radicles or caps are shown.
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