Molecular and General Genetics 256: 661-673 (1997)

Transcripts at the mating type locus of Cochliobolus heterostrophus

Gerhard Leubner-Metzger, Benjamin A. Horwitz, Olen C. Yoder, B. Gillian Turgeon

Fig. 7 mating type

Fig. 7 5' cDNA sequences aligned with the MAT-2 genomic DNA sequence. Primer sequences are indicated by thin arrows; intron 5' and 3' splice signals and the putative branch sequences are in bold; MAT- specific ORF and uORF nucleotide sequences are boxed; uORF amino acid sequences are shaded; HindIII and PstI restriction enzyme sites are underlined. Sequences were obtained from partial cDNAs GL23/3 (Type I), GL20/3 (Type II), GL20/3 (Type III), GL17/GL5 (Type I or II), and GL13/GL5 (Type III) (Figs. 5 and 6B); the genomic sequence is from Turgeon et al. (1993) with additional data derived from sequencing of the genomic GL25/3 DNA. Note that, in each of the three types of MAT-2 cDNAs, the idiomorph-specific 55-bp intron is spliced out. In Type I, represented by cDNA GL23/3, both the 79-bp and the 63-bp introns are spliced out. A proportion of the Type I transcripts start upstream of the PstI site near primer GL23. For Types II and III, represented by GL20/3 cDNAs of di?erent sizes, the 79-bp intron is not spliced out. Some of these transcripts start within the 79-bp intron sequence near primer GL20. Sequencing con®rmed that in Type II cDNA the 63-bp intron is spliced out, while it is still present in Type III. Approximate transcription starts are indicated by horizontal bars attached to arrows

Article in PDF format (540 KB) Abstract          Fig. 1          Fig. 2          Fig. 3          Fig. 4
Introns            Fig. 5           Fig. 6          Fig. 7          Fig. 8

The Seed Biology Place
Copyright © Gerhard Leubner 2000
Webdesign Gerhard Leubner 2000
Best viewed with browser version 4 and 800x600 pixel
This page was last updated on 24 March, 2011