Molecular and General Genetics 256: 661-673 (1997)

Transcripts at the mating type locus of Cochliobolus heterostrophus

Gerhard Leubner-Metzger, Benjamin A. Horwitz, Olen C. Yoder, B. Gillian Turgeon

Mating type Fig. 2A-C RNA blot analysis of MAT-1 and MAT-2 specific transcripts.

Poly(A)+ RNAs (20 µg per lane) from MAT-1 strain C5 (lanes 1), MAT-2 strain C3 (lanes 2), or strains C5 plus C3 (lanes 1 + 2) grown in MM were analysed with the following probes.

A MAT-1 idiomorph-specific PCR fragment #3 (Fig. 1).
B MAT-2 idiomorph-specific PCR fragment #2 (Fig. 1).
C Ethidium bromide- stained gel showing RNA size markers (lanes labeled RNA, sizes indicated on the right) compared with lambda DNA digested with HindIII (lane labeled k, sizes indicated on the left).

The MAT-1 probe detects a 2.2± 0.1-kb transcript (A), the MAT-2 probe detects a 2.1±0.1-kb transcript (B). Both transcripts were detected in poly(A)+ RNA from a co-culture of strains of both mating types (A, B). No signals were found when MAT-1 RNA was probed with a MAT-2 specific probe and vice-versa.

Article in PDF format (540 KB) Abstract          Fig. 1          Fig. 2          Fig. 3          Fig. 4
Introns            Fig. 5           Fig. 6          Fig. 7          Fig. 8

The Seed Biology Place
Copyright © Gerhard Leubner 2000
Webdesign Gerhard Leubner 2000
Best viewed with browser version 4 and 800x600 pixel
This page was last updated on 24 March, 2011