Molecular and General Genetics 256: 661-673 (1997)

Transcripts at the mating type locus of Cochliobolus heterostrophus

Gerhard Leubner-Metzger, Benjamin A. Horwitz, Olen C. Yoder, B. Gillian Turgeon

Figure 3 mating type Fig. 3A-C RNA blot analysis showing that transcription of the MAT genes is regulated by the composition of the culture medium.

Poly(A)+ RNAs from strain C5 (MAT-1; lanes 1) or C4 (MAT-2; lanes 2) grown in either MM or CM were probed with
(A) MAT-1 specific probe #3 (Fig. 1),
(B) MAT-2 specific probe #2 (Fig. 1) or
(C) the constitutively expressed GPD1 gene (which controls for amounts of RNA loaded).
Neither the MAT-1 nor the MAT-2 transcript was detectable when cultures were grown in CM.

Article in PDF format (540 KB) Abstract          Fig. 1          Fig. 2          Fig. 3          Fig. 4
Introns            Fig. 5           Fig. 6          Fig. 7          Fig. 8

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