Molecular and General Genetics 256: 661-673 (1997)

Transcripts at the mating type locus of Cochliobolus heterostrophus

Gerhard Leubner-Metzger, Benjamin A. Horwitz, Olen C. Yoder, B. Gillian Turgeon

Fig. 1A, B DNA probes used for transcript mapping. At the top of each panel are restriction maps of the chromosomal MAT-1 (A) and MAT-2 (B) loci. The stippled (A) and shaded (B) boxes indicate the idiomorphs; the cross-hatched boxes denote putative introns. Below each map the broken bars indicate fragments used as probes in Figs. 2, 3, 4, 9; the large arrows indicate the MAT-specific ORFs; and the small arrows indicate the positions of primers listed in Table 1. Most probes were obtained by digesting plasmids carrying MAT-1 or MAT-2 DNA (Materials and methods). Others (#2, #3 and #44) were generated by PCR with primers (SKCM2-1/SKCM2-2, SKCM1-1/ SKCM1-2, and F2/GL2, respectively). Dotted lines designate regions not drawn to scale. Only some restriction enzyme sites are shown 662

Article in PDF format (540 KB) Abstract          Fig. 1          Fig. 2          Fig. 3          Fig. 4 Introns            Fig. 5           Fig. 6          Fig. 7          Fig. 8

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