Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK (KL, PK)
Wageningen Seed Lab, Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands (BD, LB)
Department of Molecular Plant Physiology, Utrecht University, 3584 CH Utrecht, The Netherlands (BD, LB)
University of Freiburg, Faculty of Biology, Institute for Biology II, Botany/Plant Physiology, D-79104 Freiburg, Germany (TS*, GLM*)
ARC Centre of Excellence in Plant Cell Walls, School of Botany, University of Melbourne, Parkville, Victoria 3010, Australia (BD, LB)
* Current Address: School of Biological Sciences, Royal Holloway, University of London, Bourne Building 3-30, Egham, Surrey, TW20 0EX, UK
Received July 13, 2012; Accepted September 4, 2012; Published September 6, 2012.
Supplemental Figure S1. Summary of cell wall epitope detection in Arabidopsis, Lepidium and tobacco seeds.
(ME) = micropylar endosperm, (LE/CE) = peripheral and chalazal endosperm, (Em) = embryo, (T) = testa, (M) = mucliage.
(-) = probe did not bind, (+), (++) and (+++) = relative amount of probe binding for each tissue where (+) denotes weak binding and (+++) denotes intense binding.
(a) = restricted to embryo surface / inner face of endosperm.
(b) = abundant in cotyledons. [+] and [+++] = detectable upon enzyme deconstruction of the wall.