Plant Physiology 160: 1551-1566 (2012)

Distinct cell wall architectures in seed endosperms in representatives of the Brassicaceae and Solanaceae [W][OA]

Kieran J.D. Lee, Bas J.W. Dekkers, Tina Steinbrecher, Cherie T. Walsh, Tony Bacic, Leonie Bentsink, Gerhard Leubner-Metzger, J. Paul Knox

Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK (KL, PK)
Wageningen Seed Lab, Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands (BD, LB)
Department of Molecular Plant Physiology, Utrecht University, 3584 CH Utrecht, The Netherlands (BD, LB)
University of Freiburg, Faculty of Biology, Institute for Biology II, Botany/Plant Physiology, D-79104 Freiburg, Germany (TS*, GLM*)
ARC Centre of Excellence in Plant Cell Walls, School of Botany, University of Melbourne, Parkville, Victoria 3010, Australia (BD, LB)
* Current Address: School of Biological Sciences, Royal Holloway, University of London, Bourne Building 3-30, Egham, Surrey, TW20 0EX, UK

Received July 13, 2012; Accepted September 4, 2012; Published September 6, 2012.

seed cellulose

Figure 2. In situ localization of cellulose and noncellulosic polysaccharides in medial longitudinal sections of 3-h-imbibed Arabidopsis seeds.

A and B, Whole seed sections labeled with the probes CBM3a and Calcofluor White. CBM3a binding revealed cellulose in all cell walls.
C to E, ME and CE seed regions labeled with probes LM15 XG, LM25 XG, and LM21 HM. LM15 XG was detectable in embryo but not endosperm cell walls, whereas LM25 XG was detectable in testa, endosperm, and embryo cell walls. No heteromannan (HM) was detectable in the seed. C, Cotyledons; R, radicle; T, testa. Bars = 50 mm.

Article in PDF format (1.5 MB)
Supplementary data file (2 MB)
Fig. 1         Fig. 2         Fig. 3         Fig. 4         Fig. 5         Fig. 6
Fig. 7         Fig. 8         Fig. S1       Fig. S2       Table 1    
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