Journal of Experimental Botany 63: 6325-6334 (2012)

Role of a respiratory burst oxidase of Lepidium sativum (cress) seedlings in root development and auxin signalling [W][OA]

Kerstin Müller*, Ada Linkies*, Gerhard Leubner-Metzger, Allison R. Kermode

Simon Fraser University, Department of Biological Sciences, 8888 University Drive, Burnaby BC V5A 1S6, Canada (KM, ARK)
University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, German (AL, GLM)
School of Biological Sciences, Plant Molecular Science, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK (GLM); Web: 'The Seed Biology Place' -
* equal contributions

Received 20 July 2012; Revised 11 September 2012; Accepted 21 September 2012
Advance Acess publication 23 October 2012
DOI 10.1093/jxb/ers284

Fig. 4 rboh

Fig. 4. Root phenotype of seedlings derived from Lesarboh-RNAi-knock-down lines.
(A) Root of WT seedling.
(B) Root of lesarbohBRNAiB1 seedling 5 d after completion of germination: representative of phenotype stage 1. The lower part of the root is defective in tissue organization and turned into a thin thread-like structure with a thickened root tip.
(C–E) Roots of lesarbohB-RNAiB1 seedlings, 10 d after completion of germination: representative of phenotype stage 2. The thickened root tips have fallen off, and the roots have developed irregular lateral roots and callus-like tissues.
(F) Superoxide production as measured by XTT-reduction in WT seedlings and in the RNAi-seedlings at stages 1 and 2 of the root phenotype as indicated.
(G) Expression of Lesarbohs in T2 seedlings that showed the root phenotype ("short roots") versus seedlings that have WT-like roots ('long roots'). Transcript abundance was corrected to the abundance of transcripts associated with the L. sativum standard genes Actin 7 (ACT7, HQ436350.1) and Elongation Factor 1α (EF1α, HS981853.1). Averages of 5 biological replicates ± SE are shown.

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