Journal of Experimental Botany 63: 6325-6334 (2012)

Role of a respiratory burst oxidase of Lepidium sativum (cress) seedlings in root development and auxin signalling [W][OA]

Kerstin Müller*, Ada Linkies*, Gerhard Leubner-Metzger, Allison R. Kermode

Simon Fraser University, Department of Biological Sciences, 8888 University Drive, Burnaby BC V5A 1S6, Canada (KM, ARK)
University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, German (AL, GLM)
School of Biological Sciences, Plant Molecular Science, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK (GLM); Web: 'The Seed Biology Place' -
* equal contributions

Received 20 July 2012; Revised 11 September 2012; Accepted 21 September 2012
Advance Acess publication 23 October 2012
DOI 10.1093/jxb/ers284


Fig. 2 rboh Fig. 2. Expression analysis of Lesarbohs and their Arabidopsis respiratory burst oxidase orthologues.
Transcript abundance analysis for five members of the gene family determined by qRT-PCR in different plant tissues of L. sativum and A. thaliana. Note the similarities in intensity and expression profile between the two species. Averages of three biological replicates ± SE are presented. For the imbibed seed samples, seeds were harvested after 24 h on water in continuous light. Other samples included 5-d-old seedlings (5 d post-imbibition), and mature adult plants harvested 4 weeks post-germination. No AtrbohA transcript was found in any of the stages tested. Transcript abundance was corrected to the abundance of transcripts associated with the standard genes - the constitutive genes Actin 7 (ACT7; At5g09810) and Elongation Factor 1α (EF1α; At5g60390) for Arabidopsis and the corresponding genes (ACT7, HQ436350.1 and EF1α, HS981853.1) for L. sativum. n.d.=not detectable.

Article in PDF format (1.8 MB)
Supplementary data file (2.3 MB)
Fig. 1         Fig. 2         Fig. 3         Fig. 4         Fig. 5
Fig. S1       Fig. S2
© Oxford University Press, Oxford Journals
Society of Experimental Biology

The Seed Biology Place
Copyright © Gerhard Leubner 2000

Webdesign Gerhard Leubner 2000
Best viewed with browser version 4 and 800x600 pixel
This page was last updated on 3 March, 2013