Journal of Experimental Botany 63: 5337-5350 (2012)

Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A [W][OA]

Antje Voegele, Kai Graeber, Krystyna Oracz, Danuse Tarkowská, Dominique Jacquemoud, Veronika Turecková, Terezie Urbanová, Miroslav Strnad, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, German (AV, KG, KO, DJ, GLM)
School of Biological Sciences, Plant Molecular Science, Royal Holloway, University of London, Egham, Surrey TW20 0EX, Uk (AV, KG, GLM); Web: 'The Seed Biology Place' -
Department of Plant Physiology, Warsaw University of Life Sciences-SGGW, 15 Nowoursynowska 159, 02-776, Warsaw, Poland (KO)
Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, v.v.i., Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (DT, VT, TU, MS)
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (MS)

Received March 10 2012; Revised June 11 2012; Accepted June 15 2012
Advance Acess publication July 21 2012
DOI 10.1093/jxb/ers197

Ambient water potential

Figure S3. The effect of MyA and the ambient water potential (Ψmedium) on embryo imbibition, embryo size and endosperm rupture during the germination of Lepidium sativum seeds incubated in continuous white light.
(a) Early germination: Embryo mask areas at different Ψmedium in the CON- and MyA-series during the early (3h and 8h) phase of germination. The early phase is characterized by MyA-enhanced imbibition (phase-I water uptake). Embryo sizes at the onset of testa rupture (TR) are indicated.
(b) Late germination: Time courses of endosperm rupture of seed populations incubated without (CON) or with 5x10-4 M MyA, and 0, 10, 20 or 30 mM PEG as osmoticum; Ψmedium of the different PEG concentrations are indicated.

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Fig. 1         Fig. 2         Fig. 3         Fig. 4         Fig. 5         Fig. 6         Fig. 7
Fig. S1       Fig. S2       Fig. S3       Fig. S4       Table S1    
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