Journal of Experimental Botany 63: 5337-5350 (2012)

Embryo growth, testa permeability, and endosperm weakening are major targets for the environmentally regulated inhibition of Lepidium sativum seed germination by myrigalone A [W][OA]

Antje Voegele, Kai Graeber, Krystyna Oracz, Danuse Tarkowská, Dominique Jacquemoud, Veronika Turecková, Terezie Urbanová, Miroslav Strnad, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, German (AV, KG, KO, DJ, GLM)
School of Biological Sciences, Plant Molecular Science, Royal Holloway, University of London, Egham, Surrey TW20 0EX, Uk (AV, KG, GLM); Web: 'The Seed Biology Place' -
Department of Plant Physiology, Warsaw University of Life Sciences-SGGW, 15 Nowoursynowska 159, 02-776, Warsaw, Poland (KO)
Laboratory of Growth Regulators, Faculty of Science, Palacky University and Institute of Experimental Botany AS CR, v.v.i., Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (DT, VT, TU, MS)
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Šlechtitelu 11, CZ-783 71, Olomouc, Czech Republic (MS)

Received March 10 2012; Revised June 11 2012; Accepted June 15 2012
Advance Acess publication July 21 2012
DOI 10.1093/jxb/ers197

viability assay

Figure S1. The effect of myrigalone A (MyA) on the germination of Lepidium sativum seeds imbibed in darkness.
(a) Germination kinetics of CON and seeds treated with different MyA concentrations. The times to 50% endosperm rupture are 14.9±0.1 h, 20.0±0.0 h, 27.2±1.1 h, and 223.0±2.3 h for CON, 5x10-5 M MyA, 10-4 M MyA, and 5x10-4 M MyA, respectively.
(b) Atypical germination of seeds incubated in the presence of MyA in darkness.
(c) Tetrazolium viability assay of embryos from seeds incubated in darkness. Embryos were excised from seeds prior to endosperm rupture and incubated in tetrazolium solution for 30 min. CON = 0.35% (v/v) methanol.

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Fig. 1         Fig. 2         Fig. 3         Fig. 4         Fig. 5         Fig. 6         Fig. 7
Fig. S1       Fig. S2       Fig. S3       Fig. S4       Table S1    
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