Planta 235: 137-151 (2012)

Dose- and tissue-specific interaction of monoterpenes with the gibberellin-mediated release of potato tuber bud dormancy, sprout growth and induction of α-amylases and β-amylases

Sonja Rentzsch • Dagmara Podzimska • Antje Voegele • Madeleine Imbeck • Kerstin Müller§ • Ada Linkies* • Gerhard Leubner-Metzger*
*shared corresponding authors
University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place'
§Department of Biological Sciences, Simon Fraser University, 9 8888, University Drive, Burnaby BC, V5A 1S6, Canada

Received 11 June 2011; Accepted 5 August 2011; Published online 20 August 2011
DOI: 10.107/s00425-011-1501-1

amylase structure

Fig. 4   Molecular phylogenetic analysis of the potato α-amylase gene family reveals clustering into two distinct groups: α-AMY1 and α-AMY2.

The members of the two potato α-amylase groups α-AMY1 and α-AMY2 (phylogenetic tree on the left) differ among each other as presented for the pairwise comparison (table on the right) of their protein sequence identities and pI values (in brackets behind the names). Note that based on these values the α-AMY2 group contains highly conserved members as based on the sequence identities of the 'high-pI' proteins.

Alignment of deduced a-amylase amino acid sequences and characteristic domains as defined by InterPro database. 'M' and the arrow designate a conserved methionine in α-AMY1- and α-AMY2-proteins, which for the α-AMY1-proteins is the N-terminus.

Alignment of α-amylase cDNA sequences and the deduced open reading frames (long arrows). The positions for the group-specific primers for the semi-quantitative RT-PCR are indicated as triangles. Note that the forward primer for the α-AMY2-group is located within the coding sequence while for the α-AMY1-group it is located in a conserved region of the 5' untranslated cDNA region; the small arrow indicates the position of the encoded conserved methionine and the ca. 100-bp sequence downstream of the arrow does not allow the design of a forward primer that distinguishes between the two α-amylase groups.

Accession numbers to GenBank for the potato α-amylases: StAMY1a1 (JK036076), Amy21 (M81682, identical with A21345), StAMY2a1 (JK036077), Amy23 (M79328, identical with A21341), amyA2 (GU134783), amyA1 (GQ406048, identical with A21343 and with MICRO.10377.C2 of Hartmann et al., 2011).

Article in PDF format (0.9 MB)
Fig. 1         Fig. 2         Fig. 3         Fig. 4         Fig. 5
© Springer-Verlag Berlin Heidelberg

The Seed Biology Place
Copyright © Gerhard Leubner 2000

Webdesign Gerhard Leubner 2000
Best viewed with browser version 4 and 800x600 pixel
This page was last updated on 4 January, 2012