Plant and Cell Physiology 47: 864-877 (2006)
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Endosperm-limited Brassicaceae seed germination: Abscisic acid inhibits embryo-induced endosperm weakening of Lepidium sativum (cress) and endosperm rupture of cress and Arabidopsis thaliana
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Kerstin Müller, Stefanie Tintelnot, Gerhard Leubner-Metzger
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Institut für Biologie II (Botanik/Pflanzenphysiologie), Albert-Ludwigs-Universität Freiburg,
Schänzlestr. 1, D-79104 Freiburg i. Br., Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de
Received April 3, 2006; accepted May 3, 2006
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Figure 6. Direct measurement of Lepidium sativum endosperm weakening of imbibed seeds by the puncture force method. Micropylar seed halves were dissected from whole seeds imbibed (continuous light, 18 °C) for 8 h, 18 h or 120 h without (control; CON) or with hormones (ABA and/or GA4+7 in the concentrations indicated) added to the medium. Mean values ± SE of at least 40 endosperm caps are presented. Note that all these seeds had completed testa rupture, but had intact endosperm. The numbers above the columns represent the percentage of seeds with ruptured endosperm in the corresponding seed populations.
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