Plant and Cell Physiology 47: 864-877 (2006)

Endosperm-limited Brassicaceae seed germination: Abscisic acid inhibits embryo-induced endosperm weakening of Lepidium sativum (cress) and endosperm rupture of cress and Arabidopsis thaliana

Kerstin Müller, Stefanie Tintelnot, Gerhard Leubner-Metzger

Institut für Biologie II (Botanik/Pflanzenphysiologie), Albert-Ludwigs-Universität Freiburg, Schänzlestr. 1, D-79104 Freiburg i. Br., Germany, Web: 'The Seed Biology Place'

Received April 3, 2006; accepted May 3, 2006

ABA biosynthesis mutant aba1, cyp707a1, cyp707a2 eed germination

Figure 4. 
The effect of ABA biosynthesis mutations on the time courses of testa rupture (A) and endosperm rupture (B) of Arabidopsis thaliana. Wild type (WT) seeds were compared with seeds of the ABA-deficient aba1-5 mutant and of the cyp707a1 and cyp707a2 mutants, which are impaired in ABA degradation. The incidence of testa and endosperm rupture was scored with time after the start of incubation without (control; CON) or with 1 µM ABA added to the medium. The incubation was in continuous light at 24 °C. Mean values ± SE from two independent experiment with at least three plates with 50 seeds each are presented.

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   Abstract    Fig. 1               Fig. 2
Fig. 5               Fig. 6
Fig. 3               Fig. 4
Fig. 7               Table 1
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