Plant Journal 23: 215-221 (2000)

Sense transformation reveals a novel role for class I ß-1,3-glucanase in tobacco seed germination

Gerhard Leubner-Metzger and Frederick Meins, Jr.

ABA-inducible ßGLU I overexpression promotes ABA-delayed endosperm rupture of afterripened seed


Figure 1. The effect of 10 ÁM ABA on the time course of endosperm rupture and ßGLU accumulation in afterripened S1 seeds of independent sense ßGLU I (TKSG7) and empty-vector (TCIB1) transformants. (a) and (c) The incidence of endosperm rupture expressed as percent of 100-150 seeds scored with time after the start of imbibition in continuous light without (a) and with (c) 10 ÁM ABA added. Segregating seed populations of lines carrying one (TKSG7-43), two (TKSG7-34 and TKSG7-42), and three or more (TKSG7-10) transgene loci were used. Means of two replicates from one experiment are presented. Similar results were obtained in a second experiment. Error bars indicate the largest ▒ SE value. (b) and (d) The ßGLU enzyme activities of imbibed TKSG7 and TCIB1 seed populations without (b) and with (d) 10 ÁM ABA. Error bars (b) indicate the largest ▒ SE values of lines TKSG7-10, TKSG7-34, and TKSG7-42 (upper corner), and of lines TCIB1 and TKSG7-43 (lower corner). Error bars (d) indicate individual ▒ SE values (TKSG7 lines) and the largest ▒ SE value (TCIB1 lines). Note the difference in scales of (b) and (d).
© Leubner-Metzger and Meins, Plant J 23: 215-221 (2000)

Article in PDF format (280 KB)
Figure 1 Figure 2 Table 1               Glucanase constructs
© Blackwell Science
http://www.blackwell-science.com
 

The Seed Biology Place
Copyright © Gerhard Leubner 2000
Contact: gerhard.leubner@biologie.uni-freiburg.de
Webdesign Gerhard Leubner 2000
Best viewed with browser version 4 and 800x600 pixel
This page was last updated on 27 March, 2011

Copyright Notice and Website Usage Disclaimer