Plant, Cell and Environment 26: 661-671 (2003)

Calcium requirement for ethylene-dependent responses involving 1-aminocyclopropane-1-carboxylic acid oxidase in radicle tissues of germinated pea seeds

Figure 4. Effects of calcium antagonists on the ethylene-mediated induction in the radicle of germinated pea seeds of (a) in vivo ACO enzyme activity and (b) 36 kDa ACO antigen accumulation.

The 48 h-imbibed pea seeds were pre-incubated for 2 h in the presence or absence of the calcium antagonists followed by a further incubation for 8 h exposure to 30 mL/L ethylene in the presence or absence of the calcium antagonists as described in Fig. 3; RR (100 mM), W-7 (100 mM), Li (50 mM), Ca (10 mM), Neo (50 mM). Subsequently the in vivo ACO of embryonic axes was measured and immunoblot analyses was performed with protein extracts from radicles as described in Fig. 1.