© Petruzzelli et al. Plant, Cell and
Environment 26: 661-671(2003)
Figure 5. Effects of calcium antagonists on root
hair formation and elongation growth of ethylene-treated germinated pea
seeds. 48-h imbibed pea seeds were preincubated for 2 h in presence
or absence of the calcium antagonists followed by a further incubation
for 8 h with exposure to 30 µl/l ethylene in presence or absence
of the calcium antagonists. The cell differentiation zone (dz) where the
root hairs normally form, the cell elongation zone (ez) where ethylene-induced
ectopic root hairs form and the root tip (rt) are indicated. Estimations
for the relative root hair amount ('+' = control radicle dz amount, '++'
and '+++' = increased amount, '(+)' = slightly decreased amount, '-' =
absence of root hairs) and length are presented in the right part of the
(A) Control radicle without ethylene treatment; Note that root
hairs are present in the dz, but not in ez, and are only visible in the
8 h-incubated radicles at higher magnification.
(B) Ethylene-treated radicle with ectopic root hair formation.
(C) Ethylene plus 300 µl/l NBD (2,5-norbornadiene).
(D) 10 mM CaCl2 without ethylene treatment.
(E) Ethylene plus 10 mM CaCl2.
(F) Ethylene plus 1 mM LaCl3.
(G) Ethylene plus 50 µM RR.
(H) Ethylene plus 50 mM LiCl.
(I) Ethylene plus 50 mM LiCl plus 10 mM CaCl2.