Plant, Cell and Environment 26: 661-671 (2003)

Calcium requirement for ethylene-dependent responses involving 1-aminocyclopropane-1-carboxylic acid oxidase in radicle tissues of germinated pea seeds

Figure 3. Effects of calcium antagonists on the regulation by ethylene of Ps-ACO1 and Ps-ACS2 transcript levels in the embryonic axes of germinated pea seeds.

The 48 h-imbibed pea seeds were pre-incubated for 2 h in the presence or absence of the calcium antagonists followed by a further incubation for 8 h with 30 mL/L ethylene in presence or absence of the calcium antagonists. Subsequent RNA blot analyses was as described in Fig. 1.

The effects of the following substances were investigated, as indicated:
(a) different CaCl2 concentrations without ethylene treatment.
(b) Ethylene plus the membrane-impermeable Ca2+ chelator EGTA (5 mM) or the Ca2+ channel inhibitors LaCl3 (1 mM) and verapamil (Ver, 100 mM).
(c) Ethylene plus the Ca2+ channel inhibitor ruthenium red (RR).
(d) Ethylene plus the CaM-binding inhibitor W-7.
(e, f) Ethylene plus the inhibitors of the PI cycle neomycin and LiCl.

A representative experiment from three independent experiments with the same result is presented