Journal of Experimental Botany 61: 491-502 (2010)

Peroxidases identified in a subtractive cDNA library approach show tissue-specific transcript abundance and enzyme activity during seed germination of Lepidium sativum [W][OA]
Ada Linkies, Uta Schuster-Sherpa, Stefanie Tintelnot, Gerhard Leubner-Metzger, Kerstin Müller
University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' http://www.seedbiology.de

Received August 27, 2009; revised October 6, 2009; accepted October 12, 2009
Advance Acess publication 30 October 2009
DOI 10.1093/jxb/erp318
Table 1. Homozygous Arabidopsis SALKlines used in the germination screen.
Lines in bold print were used in the detailed germination screen. '–' designates lines for which endosperm rupture is reached later than for WT, '0' is equal to WT, '+' endosperm rupture earlier than WT; nd = not determined. S = cold stratified, ABA = 1 µM ABA, incubation was in continuous light. AGI locus identifier in bold means that lines were selected for further investigation.

AGI locus Protein SALK number Insert location Germination fresh seeds Germination after-ripened seeds
        18 °C 24 °C 24 °C 24 °C 18 °C 24 °C 24 °C 24 °C
            S ABA S     S ABA S
AT1G01300 aspartyl  protease SALK_021485.37.45.x exon - - - - - 0 - -
AT1G07890 APX01 SALK_000249.38.45.x exon           +    
AT1G64970 G-TMT (a) SALK_072105.54.50.x promoter - - 0 0 - - 0 -
AT1G66200 GSR2 SALK_102291.50.50.x exon nd 0 nd 0 nd 0 nd 0
AT2G18980 Prx16 SALK_028328.55.00.x exon   +       +    
AT2G34040 API5 SALK_008073.51.20.x exon nd nd nd nd - 0 0 0
AT2G36530 LOS2 SALK_100086.52.65.x promoter - 0 0 0 - 0 - 0
AT3G12620 PP2C SALK_016641.56.00.x exon - 0 0 - - 0 - -
AT3G13920 EIF4A1 SALK_107633.50.85.x promoter 0 0 0 - 0 0 + 0
AT3G21720 isocitrate lyase SALK_004127.35.10.x promoter 0 0 0 0 nd nd nd nd
AT3G52960 PrxIIE SALK_064512.50.75.x exon           -    
AT4G02890 UBQ14 SALK_107827.16.05.x exon - - - - - 0 0 -
AT4G05050 UBQ11 SALK_069877.55.25.x promoter - - - 0 - - - -
AT5G05750 DNAJ SALK_032572.56.00.x promoter 0 0 0 0 nd nd nd nd

(a) propagation failed, therfore this line is not included in the detailed germination analysis
Article in PDF format (780 KB)
(includes Suppl. data)
Abstract

 Figure 1         Figure 2         Figure 3
Table 1          Table 2          Suppl. data file
         		 Suppl. Figure S1
Suppl. Table S1
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