Plant Physiology 150: 1855-1865 (2009)

In vivo cell wall loosening by hydroxyl radicals during cress (Lepidium sativum L.) seed germination and elongation growth  [W][OA]

Kerstin Müller, Ada Linkies, Robert A.M. Vreeburg, Stephen C. Fry, Anja Krieger-Liszkay, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' (K.M., A.L., G.L.-M.)
The Edingburgh Cell Wall Group, Institute of Molecular Plant Sciences, The University of Edinburgh, The King's Buildings, Mayfield Road, Edinburgh EH9 3JH, United Kingdom (R.A.M.V., S.C.F.)
Commissariat à l'Energie Atomique (CEA), iBiTecS, CNRS URA 2096, Service de Bioénergétique, Biologie Structurale et Mécanisme, 91191 Gif-sur-Yvette Cedex, France (A.K.-L.)

Received April 9, 2009; accepted May 29, 2009; published June 3, 2009

Fig. 6

Figure 6.   Detection of in vivo •OH attack on maize seedling coleoptile cell walls by 3H-fingerprinting during elongation growth. (A) Representative 3H-fingerprint of labeled products from segments of slowly and rapidly elongating coleoptiles. Signal intensity in the scintillation count, that is 3H-labeling of former oxo groups, is plotted against distance from the origin after high-voltage paper electrophoresis (PE) at pH 3.5. Only the rapidly elongating coleoptiles showed an appreciable neutral peak (co-migrating with glucose), which was eluted and re-run by paper chromatography (PC). (B) Representative 3H-fingerprint of labeled neutral products from segments of rapidly elongating coleoptiles. The sample was eluted from the fraction that co-migrated with glucose during PE and re-run on PC. For abbreviations of acidic and neutral markers see Fig. 3.
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Figure 6         Supplemental Figure S1
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