Plant Physiology 138: 1538-1551 (2005)

Water Uptake and Distribution in Germinating Tobacco Seeds Investigated in Vivo by Nuclear Magnetic Resonance Imaging [w]

Bertram Manz, Kerstin Müller, Birgit Kucera, Frank Volke, Gerhard Leubner-Metzger

Fraunhofer-Institut für Biomedizinische Technik (IBMT), Arbeitsgruppe Magnetische Resonanz, Ensheimer Str. 48, D-66386 St. Ingbert, Germany, Web: (B.M., F.V.)
Institut für Biologie II (Botanik/Pflanzenphysiologie), Albert-Ludwigs-Universität Freiburg, Schänzlestr. 1, D-79104 Freiburg i. Br., Germany, Web: 'The Seed Biology Place' (K.M., B.K., G.L.-M.)

Received February 21, 2005; revised April 14, 2005; accepted Aprill 20, 2005; published June 24, 2005

Figure 4. Noninvasive in vivo MRM analyses of germinated wild-type tobacco seedlings. The proton signal is indicative for the water signal; details as in Figure 3. A, Water distribution in germinated seedlings. Top row, NMR microimages as longitudinal serial sections. Bottom three rows, Transverse serial microimages through one seedling. Small white arrows indicate the emerged root tip. B, Drawing of a germinated tobacco seedling is from Avery (1933). Note that the numbers in A indicate corresponding transverse sections shown in the drawing in B.
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Figure 1 and 2
Figure 5 and 6
Figure 3       Figure 4
Figure 7       Supplemental Figure S1
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