New Phytologist 184: 885-897 (2009)

The NADPH-Oxidase AtrbohB plays a role in Arabidopsis seed after-ripening [W]

Kerstin Müller, Anna Catharina Carstens, Ada Linkies, Miguel Angel Torres, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' (K.M., A.C.C., A.L., G.L.-M.)
Centro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, 28223 Pozuelo de Alarcón (Madrid), Spain (M.A.T.)

Received April 30, 2009; accepted July 13, 2009; published online September 15, 2009
DOI 10.1111/j.1469-8137.2009.03005.x

Fig. 6 carbonylation

Figure 6. Protein carbonylation is reduced in after-ripened seeds of the atrbohB mutant compared to WT. Immunoblots with antibodies against derivatives of carbonylated (=oxidized) proteins. Protein extracts from dry seeds and seeds after 6 h imbibition of wildtype (WT) and atrbohB (B). The seeds had been grown together and after-ripened in the same location for two years. Below the blot, a Coomassie-stain is shown as a loading control. The majority of the stained proteins most likely correspond to the very abundant globulin storage protein. MW, apparent molecular mass in kD.


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Figure 5
Figure 1         Figure 2         Figure 3         Figure 4
Figure 6         Suppl. Figures S1&S2          Suppl. Table S1
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