Proteomics 10: 406-416 (2010)

Proteomics reveal tissue-specific features of the cress (Lepidium sativum L.) endosperm cap proteome and its hormone-induced changes during seed germination [W]

Kerstin Müller, Claudette Job, Maya Belghazi, Dominique Job, Gerhard Leubner-Metzger

University of Freiburg, Faculty of Biology, Institute for Biology II, Botany / Plant Physiology, Schänzlestr. 1, D-79104 Freiburg, Germany, Web: 'The Seed Biology Place' (K.M., G.L.-M.)
Centre National de la Recherche Scientifique-Université Claude Bernard Lyon-Institut National des Sciences Appliquées-Bayer CropScience Joint Laboratory (UMR 5240), Bayer CropScience, Lyon, France (C.J., D.J.)
Centre d'Analyse Protéomique de Marseille, Institut Fédératif de Recherche Jean Roche, Marseille, France (M.B.)

Received July 29, 2009; revised November 3, 2009; accepted November 5, 2009
DOI 10.1002/pmic.200900548

Fig. 1 Mueller Proteomics 2010

Figure 1. Protein sample collection of cress seed endosperm caps and radicles at physiologically defined time points during germination.
(A) A germinating cress seed in the stage of testa rupture (the brownish testa has split, revealing the endosperm cap still covering the radicle) is shown next to two Arabidopsis seeds for size comparison. For dissection, the bit of testa attached to the cap is carefully removed and the radicle with the cap cut off at the place indicated with a red line. The radicle is then carefully removed from the cap with a dissection needle, yielding isolated radicles and caps as shown in the lower panel.
(B) The cress endosperm weakens during the germination process. Cress endosperm rupture and endosperm weakening are inhibited by abscisic acid (ABA). The tissue resistance of the endosperm was measured biomechanically as puncture force in the absence (CON; control) and presence of 10 mM ABA.
(C) List of the protein samples used for this work. For each sample, average endosperm rupture at the point of collection and tissue resistance of the endosperm (puncture force, from (B)) are listed. n.d. = not determinable, ER = endosperm rupture.

Article in PDF format (365 KB)
Supplemental data file (5 MB)

Figure 1         Figure 2         Figure 3         Figure 4
Suppl. Figures:     S1     S2     S3     S4
Suppl. Tables:      S1     S2     S3     S4
© Wiley InterScience
WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

The Seed Biology Place
Copyright © Gerhard Leubner 2000

Webdesign Gerhard Leubner 2000
Best viewed with browser version 4 and 800x600 pixel
This page was last updated on 10 February, 2010