Seed Science Research 13: 139-153 (2003)

Distinct expression patterns of ß-1,3-glucanases and chitinases during the germination of Solanaceous seeds

Luciana Petruzzelli, Kerstin Müller, Katrin Hermann, Gerhard Leubner-Metzger

Table 1. Effect of gibberellins (GA) and abscisic acid (ABA) on germination and endo-type ß-1,3-glucanase (ßGlu) enzyme activity accumulation in imbibed Solanaceous seed. Control 10 µM GA4 10 µM ABA Species Incubation conditions Time (hours) Seed state (a) Rupture (%) (b) ßGlu pkat (c) Rupture (%) ßGlu pkat Rupture (%) ßGlu pkat     Testa Endosperm   Testa Endosperm   Testa Endosperm   Cestroideae subgroup of Solanaceae Nicotiana plumbaginifolia cont. light 25 0.0 0.0 - - - 71.2 0.0 0.01 ± 0.00 0.0 0.0 - - - 45 0.0 0.0 0.01 ± 0.00 95.8 23.8 0.22 ± 0.01 0.0 0.0 0.01 ± 0.00 45 NG 100 0.0 0.19 ± 0.03 45 G 100 100 0.26 ± 0.03                     Nicotiana sylvestris cont. light 0 0.0 0.0 0.04 ± 0.01 - - - - - - - - - 0.0 0.0 0.04 ± 0.01 60 NG 100 0.0 0.20 ± 0.02 - - - - - - - - - 100 0.0 0.06 ± 0.01 90 G 100 100 0.79 ± 0.05 - - - - - - - - - 100 0.0 0.10 ± 0.01 darkness 90 NG 0.0 0.0 0.05 ± 0.01 - - - - - - - - - 0.0 0.0 0.04 ± 0.01                     Petunia hybrida cont. light 20 3.5 0.0 0.22 ± 0.01 9.7 0.0 0.19 ± 0.02 4.8 0.0 0.22 ± 0.01 40 46.5 0.0 0.21 ± 0.02 57.1 1.2 0.18 ± 0.01 47.8 0.0 0.19 ± 0.00 50 65.3 0.7 0.29 ± 0.02 68.5 3.9 0.24 ± 0.02 57.6 0.0 0.16 ± 0.01 60 75.3 29.2 0.39 ± 0.03 77.8 24.2 0.31 ± 0.02 79.9 3.1 0.19 ± 0.03 70 77.7 45.0 0.39 ± 0.02 79.7 48.1 0.35 ± 0.03 80.8 3.4 0.21 ± 0.02 70 NG 0.31 ± 0.03 - - - - - - 70 Endosperm (d) 0.45 ± 0.01 - - - 0.20 ± 0.01 70 Embryo (d) 0.37 ± 0.04 - - - 0.10 ± 0.00   Solanoideae subgroup of Solanaceae Physalis peruvianum cont. light 0 0.0 0.05 ± 0.01 0.0 0.05 ± 0.01 0.0 0.05 ± 0.01 20 0.0 0.28 ± 0.03 0.0 - - - 0.0 - - - 70 0.0 0.48 ± 0.04 0.0 0.88 ± 0.12 0.0 0.76 ± 0.09 140 65.2 2.08 ± 0.18 100 1.77 ± 0.21 0.0 1.30 ± 0.15 140 NG 0.0 1.23 ± 0.11 140 G 100 2.53 ± 0.27 a NG = not germinated; G = germinated b Germination of 100-150 seeds (20 seeds for P. peruviana) incubated at 24 °C; mean values of usually 3 samples c Mean values ± SE of enzyme activities in pkat / seed (P. peruviana) or pkat / µg protein (P. hybrida, N. plumbaginifolia, N. sylvestris)   d ßGlu activities of endosperms and embryos from control seeds (germinated) or ABA-treated seeds (ungerminated) were analysed separately

Article in PDF format (780 KB) Abstract               Fig. 1          Fig. 2          Fig. 3          Fig. 4                                  Table 1          Table 2          Table 3   © CABI Publishing http://hort.cabweb.org

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