Plant Molecular Biology 51: 597-605 (2003)

Ozone-induced gene expression occurs via ethylene-dependent and -independent signalling

Grimmig B (1,5), Gonzalez-Perez M N (1,2), Leubner-Metzger G (3,6), Vögeli-Lange R (3,7), Meins F (3), Hain R (4), Penuelas J (2), Heidenreich B (1), Langebartels C (1), Ernst D (1), Sanderman H Jr (1)

Figure 2. Effect of the ethylene response inhibitor 1-MCP on GLB promoter responsiveness towards O3 and ethylene. Tobacco plants transformed with the 1.63 kb GLB::GUS construct were exposed overnight to 1-MCP (1 μl/l) and then treated with O3 (300 nl/l) or ethylene (10 μl/l) for 8 h, while control plants were held in pollutant- free air after 1-MCP pretreatment. Samples for GUS activity measurement were taken immediately before and 24 h after onset of fumigation from opposite halves of a middle-aged leaf. Means ± SE (n = 3).

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