Journal of Experimental Botany 52: 1753-1759
(2001)
Antisense-transformation reveals novel roles for class I ß-1,3-glucanase
in tobacco seed after-ripening and photodormancy
ßGLU I transformants compared to vector-control lines (TCIB1).
| Line a | Number
of transgene loci b |
Time (hours) of 50 % rupture c | ß-1,3-glucanase
activity (pkat/seed) d |
|
| Testa | Endosperm | |||
| TCIB1-2 | 1 |
32.5 | 61.0 | 2.68 |
| TCIB1-6 | 2 |
32.0 | 59.5 | 2.43 |
| TCIB1-9 | 2 |
32.5 | 60.0 | 2.70 |
| TCIB1-10 | 1 |
33.0 | 61.0 | 2.88 |
| TGAG2-24 | 1 |
45.0 (12.5) | 73.0 (12.5) | 1.48 |
| TGAG2-50 | 1 |
37.0 (4.5) | 65.0 (4.5) | --- e |
| TGAG2-62 | 2 |
36.0 (3.5) | 66.5 (6.0) | 1.30 |
| TGAG2-66 | 1 |
38.0 (5.5) | 66.5 (6.0) | 2.80 |
| TGAG3-38 | ≥ 3 |
37.0 (4.5) | 68.5 (8.0) | --- |
| TGAG3-45 | ≥ 3 |
36.0 (3.5) | 65.5 (5.0) | --- |
| TGAG3-47 | 1 |
35.5 (3.0) | 64.5 (4.0) | 2.10 |
| TGAG3-58 | 1 |
36.0 (3.5) | 64.5 (4.0) | --- |
| TKAG4-30 | 2 |
38.0 (5.5) | 68.5 (8.0) | --- |
| TKAG4-31 | 1 |
38.5 (6.0) | 68.5 (8.0) | 1.56 |
| TKAG4-48 | 1 |
39.0 (6.5) | 68.5 (8.0) | 2.60 |
| TKAG4-50 | 1 |
41.0 (8.5) | 69.0 (8.5) | 2.30 |
 |
|
|
|
|
a Independent primary transformants of Havana 425 tobacco carrying antisense ßGLU I transgene loci (TGAG2, TGAG3, TKAG4) or empty-vector loci (TCIB1).
b As judged from the segregation of the KmR marker in S1 seeds.
c Determined from the time course of rupture for segregating S1 populations of 100-150 afterripened seeds imbibed in continuous light in control medium supplemented with antibiotics. Similar results were obtained when antibiotics were not added. The delay relative to the mean of TCIB1 lines is shown in parenthesis.
d Seeds sampled at the time of 50 % endosperm rupture.
e Not determined.
| Article in PDF format (160 KB) | Abstract Fig. 1 Fig. 2 Table 1 Table 2 Glucanase constructs |
 ©
Society for Experimental Biologyhttp://jxb.oupjournals.org/ |
|
|
The Seed Biology Place |
Webdesign Gerhard Leubner 2000 |
