Planta 215: 959-968 (2002)

Seed after-ripening and over-expression of class I ß-1,3-glucanase confer maternal effects on tobacco testa rupture and dormancy release

Gerhard Leubner-Metzger


tobacco dormancy

Fig. 3  Gibberellin-requirement of photodormancy release of fresh and after-ripened Havana-425 tobacco seeds imbibed in darkness.
Fresh seed batches (blue circles) are completely photodormant in control medium, i.e. no germination occurs during dark-imbibition. After-ripened seed batches (open symbols) were grouped into 5 equally-sized classes depending on their degree of non-photodormancy in control medium: class A 0-5 %, B 5-10 %, C 10-25 %, D 25-50 %, E 50-100 % germination during dark-imbibition, respectively.
The percentage of germination of approximately 100 seeds was scored after two weeks of imbibition in darkness in medium without (control) or with 0.01, 0.1, 1, or 4 µM GA4 added. The GA4-concentrations for 50 % photodormancy release are 0.73 µM for fresh seeds, and 0.73, 0.2, 0.1, 0.02 µM GA4 for after-ripened seed batches of the classes A, B, C, D, respectively.
© Leubner-Metzger, Planta 215: 959-968 (2002)
Article in PDF format (316 KB)
Abstract Fig. 1 Fig. 2 Fig. 3 Table 1          Maternal   crosses
© Springer

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