Planta 215: 959-968 (2002)
Seed after-ripening and over-expression of
class I ß-1,3-glucanase confer maternal effects on tobacco testa
rupture and dormancy release
Gerhard Leubner-Metzger
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Fig. 2 In situ immunohistological
localization of ßGlu I in mature, dry sense-ßGlu I tobacco seeds.
14-µm cryosections of TKSG7 seeds (a-e) were used to detect
ßGlu I as green fluorescence (a,d) with the rabbit anti-tobacco
ßGlu I antibody and fluorescence-labeled secondary antibody against
rabbit IgG (ALEXA-Fluor488); (d,e) was obtained by confocal microscopy.
Red autofluorescence of TKSG7 seeds (b,e), green fluorescence of
TCIB1 seeds (f-i) and green autofluorescence of wild-type seeds (j)
served as controls. Note that the outer testa layer of the seed was removed
before sectioning to decrease the amount of autofluorescence and that the
thin inner testa exhibits autofluorescence; endo. = endosperm; cot. = cotyledons;
rad. = radicle
© Leubner-Metzger, Planta 215:
959-968 (2002)
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