Plant Physiology 109: 751-759 (1995)

Class I ß-1,3-glucanases in the endosperm of tobacco during germination

Gerhard Leubner-Metzger, Corinne Fründt, Regina Vögeli-Lange (1), Frederick Meins, Jr.

Western glucanase tobacco seed germination

Figure 3. The time course of class I ß-1,3-glucanase antigen and mRNA accumulation during and following the germination of tobacco seed with 10-5 M ABA (ABA) and without ABA (control) added to the medium.

(a) Western-blot analysis of antigens reacting with antibody directed against tobacco class I ß-1,3-glucanase. Equal amounts of protein (150 µg) were applied to each lane. GLN I, 10 ng of tobacco class I ß-1,3-glucanase. The apparent size in kDa of immunoreactive bands is indicated.
(b) Northern-blot analysis of total RNA (30 µg/lane) using a hybridization probe for tobacco class I ß-1,3-glucanase mRNA. The size of the transcript in kb is indicated. Note the marked reduction in ß-1,3-glucanase mRNA accumulation in the ABA-treated seeds. Very similar patterns of accumulation were obtained when hybridization was quantitated with a PhosphoImager and normalized for the signal obtained with 18S rRNA used as an internal standard (data not shown).

Article in PDF format (2.5 MB) Abstract           Fig. 1          Fig. 2          Fig. 3          Fig. 4          Fig. 5          Table 1  

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