Plant Molecular Biology 38: 785-795 (1998)

Ethylene responsive element binding protein (EREBP) expression and the transcriptional regulation of class I ß-1,3-glucanase during tobacco seed germination

Gerhard Leubner-Metzger, Luciana Petruzzelli (1), Rosa Waldvogel, Regina Vögeli-Lange (2), Frederick Meins, Jr.

Ethylene promotes endosperm rupture Figure 1. The effect of ethylene treatment on ß-1,3- glucanase accumulation and germination of GLBGUS tobacco seeds in the light.

Populations of 100- 200 seeds were imbibed in continuous light either in the absence (open symbols) or presence of 100 ppm ethylene (filled symbols). Means values ± SE are presented for two independent experiments each with at least two samples (triplicate samples for 48, 54, and 63 h). When error bars are not shown, the SE values were ± 2 in (a) and ± 0.1 in (b).

(a) The incidence of endosperm rupture. The onset of endosperm rupture was consistently earlier in populations of ethylene-treated seeds in the two independent experiments.

(b) The accumulation of ß- 1,3-glucanase, expressed as pkat enzyme activity / seed and Glb-promoter activity expressed as fkat GUS / seed.

Hyperlink to drawing of the tobacco class I ß-1,3-glucanase B (GLB) gene promoter
Article in PDF format (2.1 MB) Abstract          Fig. 1          Fig. 2          Fig. 3          Table 1          GLB-promoter  

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