The Plant Journal 41: 133-145 (2005)

ß-1,3-Glucanase gene expression in low-hydrated seeds as a mechanism for dormancy release during tobacco after-ripening

Gerhard Leubner-Metzger

Institut für Biologie II (Botanik/Pflanzenphysiologie), Albert-Ludwigs-Universität Freiburg, Schänzlestr. 1, D-79104 Freiburg i. Br., Germany, Web: 'The Seed Biology Place'

Received 31 August 2004; revised 9 October 2004; accepted 13 October 2004

Figure 1.  Effect of sense- and antisense-ßGlu I transformation on the time courses of ßGlu I expression and testa rupture during tobacco seed after-ripening. 
ßGlu enzyme activities in sense-ßGlu I (TKSG7), antisense-ßGlu I (TKAG4), and 'empty vector' control seeds (TCIB1) during after-ripening; DAH = days after harvest; mature seeds were harvested at appr. DAP40 (i.e. fresh seeds at DAH0). 
Kinetics of testa rupture of fresh and after-ripened (> 6 months of air-dry storage) TCIB1, TKSG7, and TKAG4 seeds. Note that over-expression of ßGlu I in the covering layers of TKSG7 seeds caused promoted testa rupture already in the fresh state and that antisense-ßGlu I transformation prevented the after-ripening-mediated promotion of testa rupture of TKAG4 seeds. 
Promotion of GlbGus seed testa rupture during after-ripening. Time courses of testa rupture of GlbGus seeds at various DAH. The curves and corresponding DAH numbers are indicated by rainbow colors.  TR50 is the time needed to reach 50 % testa rupture. Note that these TR50 values are presented in Fig. 2D and that endosperm rupture is equally promoted by after-ripening.

Figure 2.  Time-course analyses of tobacco after-ripening investigated by air-dry storage of GlbGus seeds.
ßGlu and Chn enzyme activities measured in the same protein extracts by equally sensitive and specific radiometric assays. Further analyses of regions 1 and 2 are presented in Fig. 3.
(B) Gus enzyme activities measured in the same protein extracts. Seed moisture content expressed as % (w/w) H2O per FW.
Relative levels of ßGlu and Gus mRNAs determined by semi-quantitative RT-PCR with 18S rRNA as internal standard. Note: The same RNA samples at several DAH were analysed by TaqMan real-time RT-PCR presented in Fig. 3E
Times in h for 50 % testa rupture (TR50; Fig. 1C) or endosperm rupture as determined from kinetic analyses of imbibed seed populations. Note that a decrease in these values represents a promotion of testa or endosperm rupture.

Article in PDF format (476 KB)
Abstract Figure 3 and 4     Figure 5
© Blackwell Science

The Seed Biology Place
Copyright © Gerhard Leubner 2000
Webdesign Gerhard Leubner 2000
Best viewed with browser version 4 and 800x600 pixel
This page was last updated on 27 April, 2005