Electrophoresis 18: 2803-2810 (1997)
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Latex allergen database
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Anton Posch (1), Zhiping Chen (1), Michael J. Dunn (2), Colin H.
Wheeler (2), Arnd Petersen (3), Gerhard Leubner-Metzger (4), Xaver Baur
(1)
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(1) Research Institute for Occupational Medicine (BGFA), Bochum, Germany
(2) Cardiothoracic Surgery, National Heart and Lung Institute, Imperial
College School of Medicine, Heart Science Venter, Harefield Hospital, Harefield,
England
(3) Forschungszentrum Borstel, Division of Allergology, Germany
(4) Friedrich Miescher Institute, Box 2543, CH-4002 Basel, Switzerland
Received: 9 June 1997
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Abstract. Two-dimensional (2-D) electrophoresis followed by
immunoblotting and N-terminal protein microsequencing were used to characterize
and identify the IgE-reactive proteins of Hevea latex that are the
main cause of the latex type I allergy affecting especially health care
workers and spina bifida children. This approach generated a comprehensive
latex allergen database, which facilitated the integration of most of the
latex allergen data presented in the literature. The major latex allergens
Hev b 1, Hev b 3, Hev b 6 and Hev b 7 have been localized on our 2-D maps.
Moreover, we were able to identify six previously undescribed IgE-binding
latex proteins, namely enolase, superoxide dismutase, proteasome subunit
C5, malate dehydrogenase, triosephosphate isomerase and endochitinase. The
generated latex 2-D maps will provide valuable information to develop strategies
for the isolation of the novel IgE binding proteins in order to study the
frequency of sensitization among both risk groups. Detailed knowledge of
all proteins involved in latex allergy will allow better diagnosis of latex
allergy and to monitor the success of prevention strategies that are needed
to reduce the high prevalence of latex allergy among both risk groups.
Hyperlinks to Latex Allergen Databases:
Latex Allergens
UK Latex Allergy Database
Allergome
SDAP - Structural Database of Allergenic Proteins
Article in PDF format (540 KB) |
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